Part:BBa_K2082111:Design

Assembly of six mutator genes, each with weak promoter; terminal terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 194
  Illegal BamHI site found at 1081
  Illegal XhoI site found at 2663
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 66
  Illegal AgeI site found at 555
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3325
  

Design Notes

Intermediate part to make K1333108 ready for expression by cloning downstream of a promoter.

Source

cloning